Kev tsim cov txheej txheem ntawm xanthus sorbifolium protein

Cov kernels ntawm X. sorbifolium muaj cov roj thiab cov protein. Cov kernels muaj 58.91 feem pua ​​​​ roj, 14 hom fatty acids, thiab cov ntsiab lus ntawm unsaturated fatty acids yog siab li 94 feem pua. Cov kernels muaj 25.75 feem pua ​​​​ntawm cov protein, 18 hom amino acids, thiab cov hom amino acids tseem ceeb ua kom tiav, thiab cov amino acid qhab nia siab, nrog cov khoom noj muaj txiaj ntsig zoo.

Cov txheej txheem extraction ntawm cov nroj tsuag protein feem ntau muaj xws li dej extraction, alkali-soluble acid nag lossis daus txoj kev, organic solvent extraction method, membrane sib cais txoj kev, thiab lwm yam. , thiab ruaj khov zoo ntawm cov khoom tau txais. Txoj kev rho tawm cov protein tshwj xeeb yog tsim nyog rau kev rho tawm cov protein los ntawm cov txiv hmab txiv ntoo, noob thiab lwm yam qoob loo. Thaum siv cov txheej txheem nias los tsim Xanthos sorbifolium noob roj, cov roj seem tau muaj cov protein ntau, cov hmoov txhuv nplej siab thiab cov ntoo ntawm cov noob hnav, uas muaj kev loj hlob zoo thiab kev siv tus nqi thiab tuaj yeem siv rau cov khoom noj muaj protein ntau thiab pub. Qee qhov kev tshawb fawb tau txheeb xyuas cov amino acid muaj pes tsawg leeg ntawm X. sorbifolia protein, tshawb xyuas thiab ua kom zoo dua cov txheej txheem rau kev rho tawm thiab cais cov protein los ntawm X. Kev txhim kho thiab kev siv dav dav muab kev siv.

Ntsuas tus nqi ntawm Xantho sorbifolium roj residue qauv, ntxiv cov khoom tsis zoo nrog cov pH sib xws raws li cov khoom siv-rau-kua piv, leaching hauv dej da dej nrog sib nqus nplawm ntawm qhov kub thiab txias rau qee lub sijhawm, tom qab ntawd centrifuge. ntawm 5000r/min rau 15min ntawm chav tsev kub, muab pov tseg Tom qab deprecipitation, lub supernatant yog tsim nyog diluted thiab stained nrog Coomassie Brilliant Blue G-250 los ntsuas cov protein cov ntsiab lus thiab xam cov protein yield. Thaum ua cov kua qaub sib cais, cov protein extract tau los ntawm cov txheej txheem saum toj no tau hloov pauv mus rau pH sib txawv nrog 1mol / L citric acid kom ua tiav cov sedimentation, tom qab cov sedimentation tiav, centrifuge ntawm 5000r / min rau 15min, coj cov supernatant thiab khom rau 50mL thiab tom qab ntawd ntsuas cov ntsiab lus ntawm cov protein, suav cov protein sedimentation tus nqi; cov precipitate tau los ntawm centrifugation yog ntxuav nrog dej kom txog thaum nruab nrab, thiab cov protein purity tau txiav txim siab tom qab ziab.

Los ntawm ib qho kev sim thiab kev sim orthogonal optimization, cov txheej txheem zoo tshaj plaws rau kev rho tawm ntawm X. sorbifolium protein los ntawm alkali-soluble acid nag lossis daus txheej txheem yog: cov kua-kua piv 1:30, rho tawm pH 9, lub sij hawm rho tawm 150 min, thiab kub rho tawm. 60 degree. Muab rho tawm ob zaug nyob rau hauv cov xwm txheej no, cov protein tawm los yog 22.58 feem pua; Qhov zoo tshaj pH rau citric acid los nag los cais X. sorbifolium protein yog 4.6, thiab cov protein sedimentation tus nqi yog 94.67 feem pua. Alkali-dissolving thiab acid-precipitating txoj kev tuaj yeem ua rau kom purity ntawm X. sorbifolium protein los ntawm 38.46 feem pua ​​​​rau 84.03 feem pua, thiab qhov ua kom huv yog 2.18 npaug.